Zbtb16 has a role in brown adipocyte bioenergetics

摘要: A better understanding of the processes influencing energy expenditure could provide new therapeutic strategies for reducing obesity. As metabolic activity brown adipose tissue (BAT) and skeletal muscle is an important determinant overall adiposity, we investigated role genes that influence cellular bioenergetics in these two tissues. We screened are induced both BAT during acute adaptive thermogenesis mouse by microarray. used C57BL/6J mice as well primary immortalized adipocytes C2C12 myocytes to validate microarray data. Further characterization included gene expression, mitochondrial density, respiration substrate utilization. also a Hybrid Mouse Diversity Panel assess vivo effects on obesity body fat content. identified transcription factor Zbtb16 (also known Plzf Zfp14) being thermogenesis. overexpression led induction components thermogenic program, including involved fatty acid oxidation, glycolysis function. Enhanced expression increased number, respiratory capacity uncoupling. These were accompanied decreased triglyceride content carbohydrate utilization adipocytes. Natural variation mRNA levels multiple tissues across panel >100 strains was inversely correlated with weight Our results implicate novel adiposity vivo.