[20] Preparation of antibody-linked cytotoxic agents

摘要: Publisher Summary This chapter presents the preparation of antibody-linked cytotoxic agents. It discusses strategy linking agents to immunoglobulins so that biologically active conjugates retaining agent and antibody activities are produced. Immunoglobulins share a number distinctive structural features, while exhibiting enormous diversity compared other proteins. Distinctive features include (a) tetrameric arrangement two light (L) chains (Mr 23,000) heavy (H) 50,000-70,000) linked by disulfide bridges (b) division each dimer made up one L H chain into variable (V) (antigen binding) constant (C) regions. The V region occurs near amino terminal end immunoglobulin chain. different classes differ considerably in acid sequence C segment their chains, length, homologous domains chain, bridge pattern, degree polymerization, position, numbers, kind oligosaccharides. When or from same class species compared, is found contain an invariant characteristic species, whereas shows multiple substitutions ranging 10 60 first 110 residues.