Receptor-mediated transport of heme by hemopexin regulates gene expression in mammalian cells.
作者： J Alam , A Smith
DOI: 10.1016/S0021-9258(19)84616-1
关键词:
摘要: Abstract Hemopexin (HPX) transports heme to liver parenchymal cells, undergoes receptor-mediated endocytosis, and recycles intact. Incubation of mouse hepatoma (Hepa) cells with heme-HPX causes a rapid dose- time-dependent increase in the steady-state level oxygenase (HO) mRNA. A maximum induction 20-25-fold is achieved within 3 h after incubation 10 microM heme-HPX. This accumulation HO mRNA results primarily from increased transcription gene as judged by vitro nuclear run-on assays. In addition, transport into Hepa significantly decreases transferrin receptor (TfR) While 25-30-fold decrease amount TfR observed heme-HPX, no significant change rate was detected. These regulatory effects are not restricted hepatic but also human promyelocytic HL-60 cells. first direct demonstration hemopexin regulating expression mammalian
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