ROS1 Immunohistochemistry for Detection of ROS1-Rearranged Lung Adenocarcinomas

摘要: ROS1 gene rearrangements are reported in 1% to 2% of lung adenocarcinomas (ACAs) and associated with response the multitargeted tyrosine kinase inhibitor crizotinib. can be detected using fluorescence situ hybridization (FISH); however, immunohistochemistry (IHC) for protein is a promising alternate screening modality. In this study, we examine correlation between IHC FISH describe clinicopathologic characteristics ROS1-rearranged tumors. was performed clone D4D6 on whole-tissue sections. validation cohort, compared break-apart 53 cases ACA enriched an absence known genetic alterations never-smoking status. 167 consecutive from routine molecular diagnostic practice confirmed positive results by FISH. 6 (11%) were both positive. One FISH-negative case strongly All IHC-negative negative. 2 (1.2%) had strong, diffuse expression; rearrangement both. ROS1-translocated tumors wild type EGFR, KRAS, ALK commonly solid growth mucinous/cribriform features psammomatous calcification. expression tumor cells 100% sensitive 92% specific effective tool rare but clinically important subset ACAs.