Protoplast preparation without centrifugation: plant regeneration of barley (Hordeum vulgare L.).
作者： T.J. Golds , J. Babczinsky , A.P. Mordhorst , H.U. Koop
DOI: 10.1007/BF00239890
关键词:
摘要: Using a modification of the alginate film culture technique we show that it is possible to prepare and tobacco mesophyll barley cell suspension protoplasts without centrifugation. Comparable division frequencies colony development were observed from embedded with enzyme purified by A 3 × 30 min washing regime was found be minimum time necessary remove gelled matrix. The procedure provides more gentle method for isolating protoplasts. It has additional benefit recovering all cells released starting tissue. In particular, smaller are frequently lost during conventional isolation, maintained. barley, illustrate use system plants embryogenic protoplast-derived calli cultivars Dissa Igri. Finally, using small volumes (50 μl) single aggregates used isolate
springer.com
sci-hub.se 参考文章(11)
Elfriede Schäffler, Hans-Ulrich Koop, Single cell nurse culture of tobacco protoplasts: Physiological analysis of conditioning factors Journal of Plant Physiology. ,vol. 137, pp. 95- 101 ,(1990) , 10.1016/S0176-1617(11)80017-9
Timothy James Golds, Jutta Babczinsky, Hans-Ulrich Koop, Regeneration from intact and sectioned immature embryos of barley (Hordeum vulgare L.) : the scutellum exhibits an apico-basal gradient of embryogenic capacity Plant Science. ,vol. 90, pp. 211- 218 ,(1993) , 10.1016/0168-9452(93)90242-R
A. Jähne, P. A. Lazzeri, M. Jäger-Gussen, H. Lörz, Plant regeneration from embryogenic cell suspensions derived from anther cultures of barley ( Hordeum vulgare L.) Theoretical and Applied Genetics. ,vol. 82, pp. 74- 80 ,(1991) , 10.1007/BF00231280
Hans-Ulrich Koop, Hans-Georg Schweiger, Regeneration of Plants from Individually Cultivated Protoplasts using an Improved Microculture System Journal of Plant Physiology. ,vol. 121, pp. 245- 257 ,(1985) , 10.1016/S0176-1617(85)80058-4
Hideyuki Funatsuki, Horst Lörz, Paul A. Lazzeri, Use of feeder cells to improve barley protoplast culture and regeneration Plant Science. ,vol. 85, pp. 179- 187 ,(1992) , 10.1016/0168-9452(92)90114-2
K. N. Kao, Chromosomal behaviour in somatic hybrids of soybean-Nicotiana glauca Molecular Genetics and Genomics. ,vol. 150, pp. 225- 230 ,(1977) , 10.1007/BF00268120
A. J�hne, P.A. Lazzeri, H. L�rz, Regeneration of fertile plants from protoplasts derived from embryogenic cell suspensions of barley ( Hordeum vulgare L.) Plant Cell Reports. ,vol. 10, pp. 1- 6 ,(1991) , 10.1007/BF00233022
Lutz Eigel, Hans-Ulrich Koop, Nurse Culture of Individual Cells: Regeneration of Colonies from Single Protoplasts of Nicotiana tabacum, Brassica napus and Hordeum vulgare Journal of Plant Physiology. ,vol. 134, pp. 577- 581 ,(1989) , 10.1016/S0176-1617(89)80150-6
A.P. Mordhorst, H. Lörz, Embryogenesis and development of isolated barley (Hordeum vulgare L.) microspores are influenced by the amount and composition of nitrogen sources in culture media Journal of Plant Physiology. ,vol. 142, pp. 485- 492 ,(1993) , 10.1016/S0176-1617(11)81256-3
P. A. Lazzeri, R. Brettschneider, R. Lührs, H. Lörz, Stable transformation of barley via PEG-induced direct DNA uptake into protoplasts. Theoretical and Applied Genetics. ,vol. 81, pp. 437- 444 ,(1991) , 10.1007/BF00219433