Rapid production of fertile transgenic barley ( Hordeum vulgare L.) by direct gene transfer to primary callus-derived protoplasts
作者： M. Kihara , K. Saeki , K. Ito
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摘要: Protoplasts were isolated from primary calli of barley (Hordeum vulgare L.), and an antibiotic (G418) resistance gene was introduced into these protoplasts using a polyethylene glycol (PEG) DNA uptake method. Sixty-four G418 resistant obtained in nine experiments, two plants regenerated calli. NPTII ELISA Southern analysis indicated that the expressed T0 plants. These set seed transmitted to T1 results suggest our transformation system callus-derived is useful method for generation transgenic barley.
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