Expression of epidermal Growth factor receptors on human cervical, ovarian, and vulval carcinomas

摘要: We describe the properties of two monoclonal antibodies produced to a synthetic peptide consisting residues 985 996 from cytoplasmic domain epidermal growth factor (EGF) receptor. have examined group ten human tumors including cervical, ovarian, and vulval carcinomas for expression EGF receptors by immunohistological staining using one these another antibody extracellular molecule. The were sensitive amplified enzyme system less indirect method. There was generally good correlation in intensity with reagents. Both showed strong squamous cell usually weak or heterogeneous patterns adenocarcinomas. Samples each tumor solubilized detergent analyzed presence functional immunoprecipitation autophosphorylation. Three gave labeled bands, M r 170,000, on sodium dodecyl sulfate:polyacrylamide gels. DNA extracted seven digested restriction endonucleases, fragments Southern blots probes representing domains no apparent rearrangements amplifications when compared receptor gene placental DNA. These results suggest that there is high level some tumors.