Building freshwater macroinvertebrate DNA-barcode libraries from reference collection material: formalin preservation vs specimen age
作者： Donald J. Baird , Timothy J. Pascoe , Xin Zhou , Mehrdad Hajibabaei
DOI: 10.1899/10-013.1
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摘要: AbstractAs part of its ongoing work in biomonitoring, Environment Canada’s Canadian Aquatic Biomonitoring Network (CABIN) program has assembled an expert-verified reference collection 3864 specimens 604 species freshwater macroinvertebrates. Such collections are a key resource for developing deoxyribonucleic acid (DNA) barcode library to facilitate molecular identification biomonitoring samples. We examined the problems encountered using such legacy material obtain barcodes. focused on influence specimen age and preservation history. To supplement collection, we determined time-dependent effects formalin DNA-barcode integrity 4 common arthropod taxa by controlled exposure fresh obtained from laboratory cultures. Specimens were preserved with short-term fixation followed prolonged 70% ethanol. Only 19 caddisfly larval out to...
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