Effect of Bacteriocins and Conditions that Mimic Food and Digestive Tract on Biofilm Formation, In Vitro Invasion of Eukaryotic Cells and Internalin Gene Expression by Listeria monocytogenes.

作者: Lizziane Kretli Winkelströter , Elaine C. P. De Martinis

DOI: 10.1007/S12602-013-9135-1

关键词:

摘要: In this report, Listeria monocytogenes isolates were evaluated for their ability to form biofilms, adhesion/invasion of eukaryotic cells and differential expression internalin A (inl A) gene, which is related virulence potential. The presence bacteriocins lactic acid bacteria incubation at 5 °C the main factors that influenced biofilm formation by L. monocytogenes, in comparison with BHI (control). general, adhesion invasion Caco-2 significantly lower low pH (4.5), Oxgall 0.3 %. On other hand, two (INCQS 353 Reg 26c) showed higher rates when cultivated NaCl 5 % (P < 0.05). One isolate (H-2) strongest invade cells, under selected conditions, suggesting there a relationship between For all isolates, inl gene was down-regulated bacteriocins, 0.3 %, 4.5 5 °C. Nonetheless, one (HU 471), eight times sucrose, indicating food components can increase infectiveness monocytogenes.

参考文章(47)
HAJIME TAKAHASHI, SATOKO MIYA, KAZUNORI IGARASHI, TAKAYUKI SUDA, SHINTARO KURAMOTO, BON KIMURA, Biofilm formation ability of Listeria monocytogenes isolates from raw ready-to-eat seafood. Journal of Food Protection. ,vol. 72, pp. 1476- 1480 ,(2009) , 10.4315/0362-028X-72.7.1476
ED Harlow, David Lane, A Laboratory manual Cold Spring Harbor Laboratory. ,(1989)
KALLIOPI RANTSIOU, MARIOS MATARAGAS, VALENTINA ALESSANDRIA, LUCA COCOLIN, EXPRESSION OF VIRULENCE GENES OF LISTERIA MONOCYTOGENES IN FOOD Journal of Food Safety. ,vol. 32, pp. 161- 168 ,(2012) , 10.1111/J.1745-4565.2011.00363.X
Anel Lizcano, Tiffany Chin, Karin Sauer, Elaine I. Tuomanen, Carlos J. Orihuela, Early biofilm formation on microtiter plates is not correlated with the invasive disease potential of Streptococcus pneumoniae. Microbial Pathogenesis. ,vol. 48, pp. 124- 130 ,(2010) , 10.1016/J.MICPATH.2010.01.002
Taketo Kawarai, Soichi Furukawa, Naoki Narisawa, Chisato Hagiwara, Hirokazu Ogihara, Makari Yamasaki, Biofilm formation by Escherichia coli in hypertonic sucrose media. Journal of Bioscience and Bioengineering. ,vol. 107, pp. 630- 635 ,(2009) , 10.1016/J.JBIOSC.2009.01.018
Abhinav Upadhyay, Anup Kollanoor Johny, Mary Anne Roshni Amalaradjou, Sangeetha Ananda Baskaran, Kwang Sik Kim, Kumar Venkitanarayanan, Plant-derived antimicrobials reduce Listeria monocytogenes virulence factors in vitro, and down-regulate expression of virulence genes International Journal of Food Microbiology. ,vol. 157, pp. 88- 94 ,(2012) , 10.1016/J.IJFOODMICRO.2012.04.018
Hadewig Werbrouck, An Vermeulen, Els Van Coillie, Winy Messens, Lieve Herman, Frank Devlieghere, Mieke Uyttendaele, Influence of acid stress on survival, expression of virulence genes and invasion capacity into Caco-2 cells of Listeria monocytogenes strains of different origins. International Journal of Food Microbiology. ,vol. 134, pp. 140- 146 ,(2009) , 10.1016/J.IJFOODMICRO.2009.03.022
Min Seok Chae, Heidi Schraft, Lisbeth Truelstrup Hansen, Robert Mackereth, Effects of physicochemical surface characteristics of Listeria monocytogenes strains on attachment to glass Food Microbiology. ,vol. 23, pp. 250- 259 ,(2006) , 10.1016/J.FM.2005.04.004
Maı̈wenn Olier, Sandrine Rousseaux, Pascal Piveteau, Jean-Paul Lemaı̂tre, André Rousset, Jean Guzzo, Screening of glutamate decarboxylase activity and bile salt resistance of human asymptomatic carriage, clinical, food, and environmental isolates of Listeria monocytogenes International Journal of Food Microbiology. ,vol. 93, pp. 87- 99 ,(2004) , 10.1016/J.IJFOODMICRO.2003.10.010