作者: Vahid Omidvar , Ehsan Oskoueian , Hani Nazeran , Ehsan Karimi , Armin Oskoueian
DOI: 10.5897/JMPR11.1663
关键词:
摘要: This research was carried out to evaluate the phenolics and flavonoids content of Medicago sativa leaves extract characterize profiles using reversed phase-high performance liquid chromatography (RP-HPLC). The antioxidants, anti inflammatory xanthine oxidase (XO) inhibitory activities of were also determined. obtained result showed that total value 37.0 ± 0.02 mg gallic acid equivalent (GAE)/g dry matter (DM), for 12.6 0.17 rutin equivalent/g DM. In addition, RP-HPLC analyses indicated presence acid, pyrogallol, salicylic caffeic as while detected isoflavonoid naringenin, apigenin, quercitin, myrcitin daidezin. antioxidant activity Alfalfa by diphenyl-1-picrylhydrazyl (DPPH) ferric reducing power (FRAP) method 54.42 56.71% inhibition free radicals at concentration 250 μg/ml leaves crude extract, respectively, but lower than those standards such butylated hydroxytoluene (BHT) α-tocopherol. Nitric oxide (NO) assay one potential indicator 50.99% NO µg/ml, this vitamins E C 91.52 94.13%, respectively. exhibited moderate anti-inflammatory where 50% production induced RAW 264.7 cells inhibited 147.24 extract. cell viability test [3-4,5- dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay] not toxic macrophage cells. Meanwhile, XO 51.63% µg/ml. Key words: Medicago sativa, Alfalfa, functional properties, antioxidant, anti-inflammatory, oxidase.