DNA strand breaks (comet assay) and embryo development effects in grass shrimp (Palaemonetes pugio) embryos after exposure to genotoxicants.

摘要: Grass shrimp embryos develop in egg sacs (stages 1-10) attached to the female for 14-20 days after which they 'hatch' from into a swimming zoea stage (stage 11). Until emerge sacs, depend on lipids and lipovitellin stored within egg. The percent of hatch exposure toxicants relative controls was basis an embryo development assay. Exposure chromium(III) chloride, sodium chromate, mercuric 2-methyl-1,2-naphthoquinone (MNQ) resulted reduced hatching rate. In addition effects development, DNA strand damage tests were carried out contaminant-exposed embryos, using single-cell electrophoresis method often referred as comet Development 4 more affected by MNQ than 7 embryos. rates stages exposed (172 micrograms/l) 0 90%, respectively. damage, measured tail moments, 3.4 4.4, Thus, early prevented full while later not affected. It may be that repair systems are efficient thus damaged affects development.