DUSP1 induces apatinib resistance by activating the MAPK pathway in gastric cancer.

摘要: Dual-specificity phosphatase-1 (DUSP1) is an oncogene that associated with cancer progression following drug resistance. In order to investigate the potential relationship between DUSP1 and apatinib resistance in gastric cells, we preformed many assays study this problem. gene was detected by RT-qPCR assay, proteins MAPK pathway were quantified western blot CCK-8 flow cytometry Hoechest 33342 stain performed detect of cell cycles apoptosis, respectively. Immunohistochemical staining used discover expression protein patients' tumor or paratumor tissues. It found (Apa)-resistant (GC) cells showed increased DUSP1, whereas knockdown resistant resensitized these Apa. The restored sensitivity Apa result inactivation mitogen-activated kinase (MAPK) signaling induction apoptosis. vitro use combination a inhibitor, triptolide, exerted significant effects on inhibiting growth inhibition, apoptosis via signaling. patients who did not undergo chemotherapy targeted therapy, adjacent tissues higher when compared observed addition, early stages GC than advanced stages. survival rate patients. Therefore, may be responsible for resistance, serve as biomarker efficacy. conclusion, inducing downregulation promising strategy overcome