Splenocytes Can Replace Chimeric Cells and Maintain Allograft Tolerance

摘要: BACKGROUND The induction of donor-specific tolerance (DST) has recently attracted widespread attention as a new approach to facilitate engraftment without using immunosuppressants. One way in which induce DST is establish chimeric state that allows donor-derived cells exist within recipient. This study aims investigate whether splenocytes can be used maintain chimerism and prolong graft survival. METHODS Mixed bone marrow (BM) was established this model by lethally irradiating C3H mice on day 0, transplanting BM from B6D2F1 into them. Skin grafts C57BL/6 were transplanted 30. On 60, (group A), B) B6C3F1 C) administered the mice. class I major histocompatibility complex (MHC) type, percentage peripheral blood, survival skin assessed. RESULTS After splenocyte infusion, BM-derived eliminated periphery group A (86.2+/-5.9% 0.04+/-0.03%, P=0.0008), B6D2F1-derived increased quantity an allochimera B (83.7+/-7.2% 99.6+/-0.2%, P=0.021), C B6C3F1-derived significantly level 77.8% at 180 days after infusion (P=0.014), thereby maintaining chimerism. groups survived for least 200 (P=0.0003 P=0.0001, respectively). CONCLUSIONS Chimerism arising with partial MHC match maintenance specific immunotolerance