The bioanalysis of vinorelbine and 4-O-deacetylvinorelbine in human and mouse plasma using high-performance liquid chromatography coupled with heated electrospray ionization tandem mass-spectrometry.

摘要: A sensitive, specific and efficient high-performance liquid chromatography/tandem mass spectrometry assay for the simultaneous determination of vinorelbine its metabolite 4-O-deacetylvinorelbine in human mouse plasma is presented. Heated electrospray ionization was applied followed by tandem spectrometry. 50 µL aliquot protein precipitated with acetonitrile–methanol (1:1, v/v) containing internal standard vinorelbine-d3 20 µL volumes were injected onto HPLC system. Separation achieved on a 50 × 2.1 mm i.d. Xbridge C18 column using isocratic elution 1 mm ammonium acetate–ammonia buffer pH 10.5–acetonitrile–methanol (28:12:60, v/v/v) at flow rate 0.4 mL/min. The run time 5 min. quantifies both from 0.1 to 100 ng/mL sample only 50 µL. Mouse samples can be quantified calibration curves prepared plasma. Validation results demonstrate that accurately precisely presented method. now use support (pre-)clinical pharmacologic studies humans mice. Copyright © 2009 John Wiley & Sons, Ltd.