Interaction between a geminivirus replication protein and origin DNA is essential for viral replication.
作者： E.P. Fontes , P.A. Eagle , P.S. Sipe , V.A. Luckow , L. Hanley-Bowdoin
DOI: 10.1016/S0021-9258(17)37216-2
关键词:
摘要: The geminivirus, tomato golden mosaic virus (TGMV), encodes one protein, AL1, that is absolutely required for viral DNA replication. AL1 interacts with the TGMV genome by binding specifically to origin of We have investigated nature and significance AL1/origin interactions in vitro vivo using competitive transient replication assays. Competition assays established a 13-base pair (bp) element (5'-GGTAGTAAGGTAG) containing two 5-bp direct repeat motifs separated 3-bp central core constitutes high affinity site. DNAs intact 3' sequences plus (TAAGGTAG ccTAGTAAGGTAG) were stronger competitors than 5' (GGTAGTAA GGTAGTA-AccTAG), thereby demonstrating differently motifs. Replication tobacco protoplasts site an essential cis-acting No was detected mutations either recognition sequence when provided trans from plant gene expression vector. In contrast, mutation motif (ccTAGTAAGGTAG) replicated both AL3, protein involved accumulation, trans. (GGTAGTAAccTAG) presence AL3. results suggest step replication, while may serve enhance
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