Preparation, purification and characterization of alginate oligosaccharides degraded by alginate lyase from Pseudomonas sp. HZJ 216

作者: Liyan Li , Xiaolu Jiang , Huashi Guan , Peng Wang

DOI: 10.1016/J.CARRES.2011.01.023

关键词:

摘要: Alginate lyase which was purified from the fermentation solution of marine bacteria Pseudomonas sp. HJZ216 applied to hydrolyze algae alginate. Six oligosaccharides, including di- and trisaccharides, were isolated through anion exchange chromatography. The oligosaccharide structures elucidated based on electrospray ionization-mass spectrometry (ESI-MS) 2D NMR spectra analysis.

参考文章(19)
Xiaoke Hu, Xiaolu Jiang, Hueymin Hwang, Shiliang Liu, Huashi Guan, Promotive effects of alginate-derived oligosaccharide on maize seed germination Journal of Applied Phycology. ,vol. 16, pp. 73- 76 ,(2004) , 10.1023/B:JAPH.0000019139.35046.0C
Takeshi YOKOSE, Toru NISHIKAWA, Yoshiko YAMAMOTO, Yasuhiro YAMASAKI, Kenichi YAMAGUCHI, Tatsuya ODA, Growth-promoting effect of alginate oligosaccharides on a unicellular marine microalga, Nannochloropsis oculata. Bioscience, Biotechnology, and Biochemistry. ,vol. 73, pp. 450- 453 ,(2009) , 10.1271/BBB.80692
Q‐D An, G‐L Zhang, H‐T Wu, Z‐C Zhang, G‐S Zheng, L Luan, Y Murata, X Li, None, Alginate-deriving oligosaccharide production by alginase from newly isolated Flavobacterium sp. LXA and its potential application in protection against pathogens Journal of Applied Microbiology. ,vol. 106, pp. 161- 170 ,(2009) , 10.1111/J.1365-2672.2008.03988.X
Xiaoke Hu, Xiaolu Jiang, Hueymin Hwang, Shiliang Liu, Huashi Guan, Antitumour activities of alginate-derived oligosaccharides and their sulphated substitution derivatives European Journal of Phycology. ,vol. 39, pp. 67- 71 ,(2004) , 10.1080/09670260310001636695
Gregory L. Cote, Larry H. Krull, Characterization of the exocellular polysaccharides from Azotobacter chroococcum Carbohydrate Research. ,vol. 181, pp. 143- 152 ,(1988) , 10.1016/0008-6215(88)84030-8
G. L. Miller, Use of Dinitrosalicylic Acid Reagent for Determination of Reducing Sugar Analytical Chemistry. ,vol. 31, pp. 426- 428 ,(1959) , 10.1021/AC60147A030
Sherry X Ci, Tanya H Huynh, Leslie W Louie, Andrew Yang, Bridget J Beals, Nilesh Ron, Wen-Ghih Tsang, Patrick Soon-Shiong, Neil P Desai, Molecular mass distribution of sodium alginate by high-performance size-exclusion chromatography Journal of Chromatography A. ,vol. 864, pp. 199- 210 ,(1999) , 10.1016/S0021-9673(99)01029-8
Tina Salomonsen, Henrik Max Jensen, Flemming Hofmann Larsen, Stefan Steuernagel, Søren Balling Engelsen, Alginate monomer composition studied by solution- and solid-state NMR – A comparative chemometric study Food Hydrocolloids. ,vol. 23, pp. 1579- 1586 ,(2009) , 10.1016/J.FOODHYD.2008.11.009
Mahesh Chhatbar, Ramavatar Meena, Kamalesh Prasad, AK Siddhanta, None, Microwave assisted rapid method for hydrolysis of sodium alginate for M/G ratio determination Carbohydrate Polymers. ,vol. 76, pp. 650- 656 ,(2009) , 10.1016/J.CARBPOL.2008.11.033