A Downmodulated MicroRNA Profiling in Patients with Gastric Cancer.
作者： Tao Zhang , Chang Liu , Shi Huang , Yuanping Ma , Jiansong Fang
DOI: 10.1155/2017/1526981
关键词:
摘要: Objective. Here, we aim to investigate the microRNA (miR) profiling in human gastric cancer (GC). Methods. Tumoral and matched peritumoral specimens were collected from 12 GC patients who underwent routine surgery. A high-throughput miR sequencing method was applied detect aberrantly expressed miRs a subset of 6 paired samples. The stem-loop quantitative real-time polymerase chain reaction (qRT-PCR) assay subsequently performed confirm results remaining also validated vitro three cell lines (BGC-823, MGC-803, GTL-16) normal epithelial line (GES-1). Results. approach detected 5 differentially miRs, hsa-miR-132-3p, hsa-miR-155-5p, hsa-miR-19b-3p, hsa-miR-204-5p, hsa-miR-30a-3p, which significantly downmodulated between tumoral tissues. Most further confirmed by qRT-PCR, while no change observed for hsa-miR-30a-3p. finding agreed with both qRT-PCR hsa-miR-132-3p. Conclusion. Together, our findings may serve identify new molecular alterations as well enrich GC.
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