Over Expression of BCL2 and Low Expression of Caspase 8 Related to TRAIL Resistance in Brain Cancer Stem Cells.
作者： Ling Qi , Kuang Ren , Fang Fang , Dong-Hai Zhao , Ning-Jiang Yang
DOI: 10.7314/APJCP.2015.16.12.4849
关键词:
摘要: Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) has been investigated as an effective agent to treat various cancers. Cancer stem cells are resistant TRAIL treatment, but the mechanism of resistance remains unknown. In this study, brain cancer were isolated by CD133 magnetic sorting, and number positive detected flow cytometry. The self-renewing capacity was examined a neurosphere formation assay, percentage cell death after treatment MTS assay. Expression DR5, FADD, caspase 8 BCL2 proteins western blot. amount enriched 71% sorting. Brain significantly increased treatment. also reduced viable decrease inhibited inhibitor or pan-caspase z-VAD (P<0.05). expressed lower levels protein higher when compared with negative Our data suggest that is related overexpression low expression which limit activation in cells.
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