Immune complex orchitis in vasectomized rabbits.

摘要: The extent of autoimmune orchitis in 24 vasectomized rabbits was explored by 5 serologic tests light and electron microscopy immunofluorescene elution immune globulins from tissue sections. 46% the had circulating antibodies against acrosomal androgens indirect immunofluorescence 37% testis tissues 14 were positive 1 or more 4 tanned red cell hemagglutination complement fixation agar gel double diffusion cryostat Rabbits with high antibody titers thickened basement membranes seminiferous tubules seen which appeared opaque sometimes layered spikes micrographs. Macrophages lymphocytes infiltrated basal some cases severe Sertoli cells degenerated disappeared. Granular rabbit IgG deposits demonstrated along lamina densa. immunoglobulins could be partially eluted sections heat salt buffer solutions then stained fluorescein-labeled antisperm antibody. Thsi reacted apical germinal sperm acrosomes. These eluates those kidneys 2 demonstrable renal C3 antigens immunofluorescence. There a direct correlation between levels amount severity histologic lesions these rabbits.