Bench-scale biosynthesis of isonicotinic acid from 4-cyanopyridine by Pseudomonas putida

摘要: Pseudomonas putida CGMCC3830 harboring nitrilase was used in isonicotinic acid production from 4-cyanopyridine. This showed optimum activities towards 4-cyanopyridine at pH 7.5 and 45°C. The half-life of P. 93.3 h, 33.9 9.5 h 30°C, 38°C, 45°C, respectively. 4-Cyanopyridine (100 mM) fully converted into within 20 min. bench-scale carried out using 3 mg resting cells per mL a 1 L system 30°C finally, 123 g L−1 were obtained 200 min without any by-products. conversion reaction suffered the product inhibition effect after tenth feeding. volumetric productivity 36.9 h−1. shows significant potential nitrile hydrolysis for production. paper is first report on biosynthesis it represents highest reported so far.