Analysis of pp60c-src in human colon carcinoma and normal human colon mucosal cells.

摘要: The tyrosine-specific protein kinase activity of pp60c-src molecules obtained from RIPA buffer lysates human colon tumor-derived cell lines is elevated over that found in normal mucosa cells. elevation the cultured tumor cells does not appear to be result overexpression suggesting specific phosphotransferase may enhanced. Cell-free translation c-src mRNA and yielded with similar levels vitro these regulated differently at a post-translational level. Analysis carcinoma revealed they possessed indistinguishable sites quantities phosphorylated serine tyrosine residues were detectably complexed other cellular proteins. activation associated an apparent increase turnover rate tyrosine-phosphates within carboxyl terminal portion derived lines.