Amplification and Expression of Protooncogenes in Human Small Cell Lung Cancer Cell Lines

摘要: Amplification and expression of 16 protooncogenes were examined in 12 established small cell lung cancer (SCLC) lines. Seven lines showed a 20- to 35-fold amplification the c-myc oncogene, 3 an 80- 130-fold N-myc one line had simultaneous c-myb oncogene. In this both oncogenes transcriptionally highly active at same time. A variant subpopulation SCLC expressed 8.5-kilobase v-fms homologous transcript high levels but without c-fms gene. All similar RNA N-ras, Ki-ras, Ha-ras, c-raf1 oncogenes. DNA amplification, however, was undetectable. The c-fes, c-fos, c-erbB very weakly transcripts c-mos, c-sis, c-erbA, c-src, c-abl not observed any SCLC-cell From these data we conclude that beyond myc myb, whose gene products are GTP binding proteins phosphokinases may also be necessary develop keep malignant state SCLC. found involved transition classic type