Plasma surface modification of electrospun fibers for adhesion-based cancer cell sorting

摘要: Personalized cancer therapies drive the need for devices that rapidly and accurately segregate cells from solid tumors. One potential sorting strategy is to populations of based on their relative strength adhesion. To investigate effect surface hydrophilicity cell phenotype adhesion, primary human breast skin fibroblasts keratinocytes MCF-7 were seeded onto air CF4 plasma-treated nanofibers followed by exposure three shear stresses (200, 275 350 dynes per cm2) 1 hour after inoculation. No difference in adhesion was measured either or plasma treated-surface: all exhibited >60% initial count a 5 minute cm2 stress. In contrast, significant between versus surfaces observed cells: 26% 6.6% remained surfaces, respectively. The ability sort this line two non-cancerous evaluated inoculating mixture types simultaneously treated culture majority removed (0.7% remained) while adhered (74 57%). Post-sorted viability morphology unchanged, preserving possibility post-separation analysis. These data suggest treatment electrospun scaffolds provides tool useful mixed population strength.