Genome relatedness among African swine fever virus field isolates by restriction endonuclease analysis
作者： Ronald D. Wesley , Ann E. Tuthill
DOI: 10.1016/0167-5877(84)90048-5
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摘要: Abstract The genome of African swine fever (ASF) virus has been characterized by restriction endonuclease analysis. ASF field isolates were propagated in pigs and viral DNA was extracted from infected pig erythrocytes. A relationship among nine based upon characteristic fragment migration patterns on agarose gels. Eight endonucleases permitted classification the into four major groups; last group further broken down three subgroups as follows: I II III IV Hinde-I Uganda Tengani Spencer Lisbon '60 Madrid '75 Dominican Republic Port-au-Prince Cameroon are remarkably stable during natural infections. obtained years apart different geographic areas yet have identical gel profiles. These indistinguishable a recent isolate. relative stability may suggest common source infection for Caribbean outbreaks.
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