Applications of nonequilibrium response spectroscopy to the study of channel gating. Experimental design and optimization.

摘要: Abstract A novel experimental technique known as non-equilibrium response spectroscopy (NRS) based on ion channel responses to rapidly fluctuating voltage waveforms was recently described (Millonas & Hanck, 1998a). It demonstrated that such can be affected by subtle details of the kinetics are otherwise invisible when conventional stepped pulses applied. As a consequence, probed in much more sensitive way supplementing techniques with measurements complex waveforms. In this paper we provide an analysis problem design and optimization We introduce some methods for determination parametric uncertainty class kinetic models particular data set. The allows characterization amount information acquired through set experiments which turn used new increase information. revisit application dichotomous noise 1998a, b), further consider applications general continuous wavelet -based controlled illustration these is provided making use simplified “toy” model potassium kinetics.