Influence of Estrogens on Mouse Uterine Epidermal Growth Factor Precursor Protein and Messenger Ribonucleic Acid

摘要: Estrogens stimulate the in vivo proliferation of epithelial cells mouse uterus. The cumulative evidence from several earlier studies suggests that mitogenic effect estrogens is mediated indirectly through a polypeptide growth factor. primary focus present investigation was to determine whether an epidermal factor (EGF) -related originates uterus immature or adult under normal altered estrogen status. Hybridization experiments revealed presence 4.7- kilobase prepro-EGF mRNA uteri CD-I mice. level this augmented at least 2-fold mice treated for 4 days with estrogen, but levels remained markedly low compared those submaxillary gland kidney. Two preparations pooled uterine luminal fluid estrogen-treated contained EGF immunoreactivity (1.2 and 1.7 ng/ml) stable response acid (50 mM acetic acid) heat. Negligible (<20 pg/uterus) detecte...