Bovine Type III Interferon Significantly Delays and Reduces the Severity of Foot-and-Mouth Disease in Cattle

摘要: Interferons (IFNs) are the first line of defense against viral infections. Although type I and II IFNs have proven effective to inhibit foot-and-mouth disease virus (FMDV) replication in swine, a similar approach had only limited efficacy cattle. Recently, new family IFNs, III IFN or IFN-λ, has been identified human, mouse, chicken, swine. We bovine IFN-λ3 (boIFN-λ3), also known as interleukin 28B (IL-28B), demonstrated that expression this molecule using recombinant replication-defective human adenovirus 5 (Ad5) vector, Ad5-boIFN-λ3, exhibited antiviral activity FMDV cell culture. Furthermore, inoculation cattle with Ad5-boIFN-λ3 induced systemic upregulation IFN-stimulated gene upper respiratory airways skin. In present study, we could be delayed for at least 6 days when were inoculated challenged 24 h later by intradermolingual FMDV. delay appearance was significantly prolonged treated aerosolization FMDV, method resembles natural route infection. No clinical signs FMD, viremia, shedding nasal swabs found Ad5-boIFN-λ3-treated animals 9 postchallenge. Our results indicate boIFN-λ3 plays critical role innate immune response To end, work represents most successful biotherapeutic strategy so far tested control