Two tumor suppressive loci on chromosome 10 involved in human glioblastomas

摘要: A number of cytogenetic and molecular analyses have revealed very frequent extensive losses regions chromosome 10 in human glioblastomas. Our recent studies demonstrated that the transfer a into glioblastoma cells resulted suppression their transformed tumorigenic phenotype. To localize suppressive region further, we isolated characterized certain hybrid had undergone chromosomal rearrangements to yield retaining only various inserted 10. One series subclones showed loss majority long arm (10q21-10qter) regained ability grow under anchorage-independent conditions, but still failed exhibit significant tumorigenicity nude mice. Another set exhibited major deletions large segments (10q21-q23; 10q26-qter), yet retained distal alleles associated with 10q24 10q26. These were identical biological characteristics hybrids containing an intact 10, exhibiting no growth soft agarose or results implicate presence two independent phenotypically on (10pter-q11 10q24-q26) are involved glioma progression.