Histamine H4 receptors in normal conjunctiva and in vernal keratoconjunctivitis

摘要: To cite this article: Leonardi A, Di Stefano Vicari C, Motterle L, Brun P. Histamine H4 receptors in normal conjunctiva and vernal keratoconjunctivitis. Allergy 2011; 66: 1360–1366. Abstract Background:  While it is known that histamine the primary mediator of ocular allergy, presence distribution are not well documented human eye. Our aim was to evaluate receptor expression keratoconjunctivitis conjunctiva. Methods:  Mucosal biopsies were obtained from healthy donors tarsal patients. Immunostaining semi-quantitative reverse-transcriptase polymerase chain reaction for H1, H2, H3, performed. also evaluated conjunctival cell cultures exposed histamine, interleukin-4, interleukin-5, interferon-γ tumor necrosis factor-α. Results:  H1 H2 slightly positive over-expressed tissues. H3 rarely present inflamed conjunctiva. In striking contrast control tissues, highly expressed all particularly by stromal inflammatory cells. Semi-quantitative demonstrated an over-expression vs Notably, five times more cultures, stimulated eight levels interleukin-4 three but only affected stimulation with these mediators. Conclusions:  Increased keratoconjunctival tissues indicate their important role pathogenesis disease. may be a target treatment allergic inflammation.