miR-34a inhibits migration and invasion by regulating the SIRT1/p53 pathway in human SW480 cells
作者： MINGGUANG LAI , GANG DU , RUIYUE SHI , JUN YAO , GENHUA YANG
关键词:
摘要: MicroRNA-34a (miR-34a) is a direct transcriptional target of p53, and downregulated in several different types cancer. However, the underlying mechanism miR-34a effects colorectal cancer not well understood. In this study, we explored role cell invasion, migration, apoptosis. Transient overexpression SW480 cells caused severe decrease migration invasion (both, p<0.05) compared to control groups. Combining transfection with 5-fluorouracil (5-FU) treatment further enhanced inhibition 5-FU alone. These cellular changes were associated upregulation acetylated‑p53 (ac-p53) p21 downregulation sirtuin 1 (SIRT1). data demonstrate that regulates expression number critical proteins involved apoptosis, proliferation response chemotherapy. summary, increases sensitivity colon through specific regulation SIRT1/p53 pathway.
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