Dynamic modeling of electrophoretically mediated microanalysis.
作者： Dale H. Patterson , Bryan J. Harmon , Fred E. Regnier
DOI: 10.1016/0021-9673(95)01247-8
关键词:
摘要: A dynamic model is presented for simulation of reaction-based chemical analysis enzymes and substrates in capillary electrophoretic systems by the methodology electrophoretically mediated microanalysis (EMMA). The mathematical utilizes mass balance expressions describing time-dependent effects electromigration, reaction, diffusional band broadening upon concentration profiles various reagent product species. implemented an iterative computer program which segmented into arrays bins storing each During time increment, electrophoresis, reaction kinetics, diffusion are calculated, concentrations stored updated. flexibility to accommodate initial conditions, sample introduction methods, voltage programming allows diverse EMMA analyses be simulated. simulated results shown good qualitative agreement with experimental data zonal injection moving boundary determinations leucine aminopeptidase as well ethanol.
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