In Vitro and In Vivo Anti-Osteoarthritis Effects of 2,3,5,4′-Tetrahydroxystilbene-2-O-β-d-Glucoside from Polygonum Multiflorum

摘要: Polygonum multiflorum Thunb. is a traditional herbal medicine that rich in polyphenols. The major compound, 2,3,5,4′-tetrahydroxystilbene-2-O-β-d-glucoside (THSG) has many pharmacological activities, such as antioxidative and free radical-scavenging properties, the abilities to reduce hyperlipidemia, prevent lipid peroxidation, protect cardiovascular system. In this study, anti-osteoarthritis (OA) effects of THSG were explored using vitro vivo models. inhibited nitric oxide (NO) prostaglandin E2 (PGE2) production inducible NO synthase (iNOS) cyclooxygenase-2 expressions by lipopolysaccharide-stimulated RAW 264.7 cells. On other hand, PGE2 iNOS matrix metalloproteinase-13 interleukin-1β-stimulated primary rat chondrocytes. Through mono-iodoacetate-induced OA model assay, reduced paw edema improved weight-bearing distribution. Therefore, anti-inflammatory activity could be applied lead compound for development an drug.