Cultured Keratinocytes on Urinary Bladder Matrix Scaffolds Increase Angiogenesis and Help in Rapid Healing of Wounds
作者： Ahmad Eweida , Manal Saad , Essam Gabr , Mona Marei , Mohammad Rafik Khalil
DOI: 10.1097/01.ASW.0000398665.51283.44
关键词:
摘要: INTRODUCTION: Urinary Bladder Matrix (UBM) is an extracellular matrix (ECM) scaffold. It now used in wound care management of partial and full-thickness wounds where conventional methods for usually fail to give satisfactory results. OBJECTIVE: In this study, the authors are comparing healing excisional New Zealand rabbits using either UBM scaffolds alone or combination with cultured keratinocytes. The were compared grossly histologically. MATERIALS AND METHODS: a comparative controlled study including 40 2 groups. Group (A) wounds: treated scaffolds, (B) examined after 1, 2, 3 weeks, histologically at end 3rd week ordinary hematoxylin-eosin staining techniques. RESULTS: All healed completely by week. Early contraction was significantly less group B. More angiogenic response evident all specimens CONCLUSION: This shows that adding keratinocytes rough surface scaffold may be beneficial reducing early improving vascularity treatment wounds.
lww.com
sci-hub.se 参考文章(23)
M J Brown, P T Pearson, F N Tomson, Guidelines for animal surgery in research and teaching. AVMA Panel on Animal Surgery in Research and Teaching, and the ASLAP (American Society of Laboratory Animal Practitioners). American Journal of Veterinary Research. ,vol. 54, pp. 1544- 1559 ,(1993)
MICHAEL R. MADDEN, JEROME L. FINKELSTEIN, LISA STAIANO-COICO, CLEON W. GOODWIN, G. THOMAS SHIRES, EILEEN E. NOLAN, JOHN M. HEFTON, Grafting of cultured allogeneic epidermis on second- and third-degree burn wounds on 26 patients. Journal of Trauma-injury Infection and Critical Care. ,vol. 26, pp. 955- 962 ,(1986) , 10.1097/00005373-198611000-00001
Tiffany L. Sellaro, Anjani K. Ravindra, Donna Beer Stolz, Stephen F. Badylak, Maintenance of hepatic sinusoidal endothelial cell phenotype in vitro using organ-specific extracellular matrix scaffolds. Tissue Engineering. ,vol. 13, pp. 2301- 2310 ,(2007) , 10.1089/TEN.2006.0437
S.F. Badylak, B. Kropp, T. McPherson, H. Liang, P.W. Snyder, Small intestinal submucosa: a rapidly resorbed bioscaffold for augmentation cystoplasty in a dog model. Tissue Engineering. ,vol. 4, pp. 379- 387 ,(1998) , 10.1089/TEN.1998.4.379
Donald O Freytes, Stephen F Badylak, Thomas J Webster, Leslie A Geddes, Ann E Rundell, Biaxial strength of multilaminated extracellular matrix scaffolds. Biomaterials. ,vol. 25, pp. 2353- 2361 ,(2004) , 10.1016/J.BIOMATERIALS.2003.09.015
Thomas W. Gilbert, Ann M. Stewart-Akers, Stephen F. Badylak, A quantitative method for evaluating the degradation of biologic scaffold materials Biomaterials. ,vol. 28, pp. 147- 150 ,(2007) , 10.1016/J.BIOMATERIALS.2006.08.022
Rae D Record, Darren Hillegonds, Cassandra Simmons, Robert Tullius, Frank A Rickey, David Elmore, Stephen F Badylak, In vivo degradation of 14C-labeled small intestinal submucosa (SIS) when used for urinary bladder repair. Biomaterials. ,vol. 22, pp. 2653- 2659 ,(2001) , 10.1016/S0142-9612(01)00007-2
Jason Hodde, Naturally Occurring Scaffolds for Soft Tissue Repair and Regeneration Tissue Engineering. ,vol. 8, pp. 295- 308 ,(2002) , 10.1089/107632702753725058
Stephen F. Badylak, Thomas W. Gilbert, Immune response to biologic scaffold materials. Seminars in Immunology. ,vol. 20, pp. 109- 116 ,(2008) , 10.1016/J.SMIM.2007.11.003
Janet E. Reing, Li Zhang, Julie Myers-Irvin, Kevin E. Cordero, Donald O. Freytes, Ellen Heber-Katz, Khamilia Bedelbaeva, Donna McIntosh, Abiche Dewilde, Susan J. Braunhut, Stephen F. Badylak, Degradation products of extracellular matrix affect cell migration and proliferation. Tissue Engineering Part A. ,vol. 15, pp. 605- 614 ,(2009) , 10.1089/TEN.TEA.2007.0425