PARAFORMALDEHYDE -GLUTARALDEHYDE AS A ROUTINE PHYTOPLANKTON FIXATIVE

作者: Diane Lazinsky , Linda Sicko-Goad

DOI: 10.1016/0047-7206(79)90026-8

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摘要: Several fixatives such as Lugol's iodine solution and osmium tetroxide traditionally have been employed in phytoflagellate studies. (LI) stains cytoplasmic components, making the algae easier to study with a light microscope while at same time it preserves their flagella. However, this fixative is not suitable for electron microscopy. Osmium (Os) also an excellent flagella (Rosowski, 1977). But due its extreme toxicity, useful shipboard sampling sample transport. Since we successfully paraformaldehyde-glutaraldehyde (PFG) modified after Karnovsky (1965) our laboratory ultrastructural cytochemical studies of cultured diatoms, attempted use routine microscopic phytoflagellates avoid toxicity problems provide samples that would be microscopy, SEM, TEM. Unialgal cultures Pandorina Euylena were present fixed by one following methods: