miR-423-5p contributes to a malignant phenotype and temozolomide chemoresistance in glioblastomas.

摘要: Background Gliomas are based on a genetic abnormality and present with dismal prognosis. MicroRNAs (miRNAs) considered to be important mediators of gene expression in glioma tissues. Methods Real-time PCR was used analyze the microRNA-423-5p (miR-423-5p) human samples normal brain tissue. Apoptosis, cell cycle, proliferation, immunostaining, transwell, vitro 2D 3D migration, chemosensitivity assays were performed assess phenotypic changes cells overexpressing miRNA-423-5p. Western blotting determine inhibitor growth 4 (ING-4)in tissues, luciferase reporter assay conducted confirm whether ING-4 is direct target miR-423-5p. identify potential signaling pathways that affected by Xenograft tumors examined vivo for carcinogenic effects miR-423-5p Results We first reported increased gliomas tumor promoter via targeting ING-4. The overexpression resulted upregulation molecules such as p-AKT p-ERK1/2. In clinical samples, dysregulated, corresponding alteration observed (P = .0207). Furthermore, strengthened angiogenesis, invasion. Finally, also GBM neurosphere formation rendered resistant temozolomide (TMZ). Conclusion This study establishes functions an oncogene tissues suppressing suggests it has therapeutic glioma.