MiR-423-5p knockdown enhances the sensitivity of glioma stem cells to apigenin through the mitochondrial pathway

摘要: This study aimed to investigate the effect of miR-423-5p on sensitivity glioma stem cells apigenin and explore potential mechanism. Previous research indicated that can effectively inhibit proliferation many cancer cells, including though our data unexpectedly showed had no cell apoptosis. As studies have reported malignant transformation progression are due an anti-glioma approach has become important direction for treatment. In this study, we found be overexpressed in tissues corresponding cells. Downregulation repressed growth but did not cause Based concept "Pharmaco-miR," further demonstrated combination knockdown a notable additive inhibiting promoting apoptosis Hoechst staining higher rates typical apoptotic morphological changes nucleus, JC-1 (5,5',6,6'-tetrachloro-1,1',3,3'-tetraethylbenzimi-dazolylcarbocya-nine iodide) revealed reduced mitochondrial membrane potential. Further mechanism is associated with shift Bax/Bcl-2 ratio, increased cytochrome c level, Apaf-1 induction, caspase-3 activation. conclusion, indicates downregulation enhances through pathway.