Region-specific astrogliosis in brains of mice heterozygous for mutations in the neurofibromatosis type 1 (Nf1) tumor suppressor

摘要: Brains from human neurofibromatosis type 1 (NF1) patients show increased expression of glial fibrillary acidic protein (GFAP), consistent with activation astrocytes (M.L. Nordlund, T.A. Rizvi, C.I. Brannan, N. Ratner, Neurofibromin and astrogliosis in (type 1) brains, J. Neuropathol. Exp. Neurology 54 (1995) 588–600). We analyzed brains transgenic mice which the Nf1 gene was targeted by homologous recombination. here that, all heterozygous analyzed, there are numbers expressing high levels GFAP medial regions periaqueductal gray nucleus accumbens. More subtle, but significant, changes number positive were observed hippocampus 60% mutant analyzed. Astrocytes elevated present at month, 2 months, 6 months 12 after birth. Most brain regions, including cerebellum, basal ganglia, cerebral cortex, hypothalamus, thalamus, cortical amygdaloid area, white matter tracts did not any gliotic changes. No evidence degenerating neurons found using de Olmos’ cupric silver stain. conclude that Nf1/nf1 provide a model to study associated 1.