SKOV3 ovarian carcinoma cells have functional estrogen receptor but are growth-resistant to estrogen and antiestrogens.
作者： Wenhui Hua , Tracy Christianson , Christian Rougeot , Henri Rochefort , Gail M. Clinton
DOI: 10.1016/0960-0760(95)00187-5
关键词:
摘要: Estrogen receptor positive ovarian cancer is often refractile to antiestrogen therapy. Here we describe the SKOV3 human carcinoma cell line as an in vitro model for estrogen and resistant cancer. While cells expressed (ER) mRNA protein at a similar level responsive T47D breast line, their growth was not estradiol (E2) inhibited by antiestrogens OH-tamoxifen ICI 164,384. The ER normal with respect apparent Kd binding E2, E2 regulation of transiently transfected ERE driven reporter gene, stimulation expression early response genes c-myc c-fos. However, exhibited no progesterone gene (PR) even after addition products HER-2/neu cathepsin D were constitutive levels that regulated E2. Therefore, resistance these may be result loss selected regulatory rather than defect activation transcriptional regulator.
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sci-hub.se 参考文章(45)
O. Bernard, S. Cory, S. Gerondakis, E. Webb, J.M. Adams, Sequence of the murine and human cellular myc oncogenes and two modes of myc transcription resulting from chromosome translocation in B lymphoid tumours. The EMBO Journal. ,vol. 2, pp. 2375- 2383 ,(1983) , 10.1002/J.1460-2075.1983.TB01749.X
H. Nawata, D. Bronzert, M.E. Lippman, Isolation and characterization of a tamoxifen-resistant cell line derived from MCF-7 human breast cancer cells. Journal of Biological Chemistry. ,vol. 256, pp. 5016- 5021 ,(1981) , 10.1016/S0021-9258(19)69359-2
Wilma M. McKoy, Karen R. Grotzinger, Elizabeth W. Chu, William R. Green, Robert C. Young, Jacqueline Whang-Peng, Alfred M. Rogan, John A. Green, Robert F. Ozols, Thomas C. Hamilton, Characterization of a Human Ovarian Carcinoma Cell Line (NIH:OVCAR-3) with Androgen and Estrogen Receptors Cancer Research. ,vol. 43, pp. 5379- 5389 ,(1983)
Robert P. C. Shiu, Richard T. Pon, Peter H. Watson, Inhibition of c-myc expression by phosphorothioate antisense oligonucleotide identifies a critical role for c-myc in the growth of human breast cancer. Cancer Research. ,vol. 51, pp. 3996- 4000 ,(1991)
Anne E. Lykkesfeldt, Per Briand, Mogens W. Madsen, Altered expression of estrogen-regulated genes in a tamoxifen-resistant and ICI 164,384 and ICI 182,780 sensitive human breast cancer cell line, MCF-7/TAMR-1. Cancer Research. ,vol. 54, pp. 1587- 1595 ,(1994)
P. Kastner, A. Krust, B. Turcotte, U. Stropp, L. Tora, H. Gronemeyer, P. Chambon, Two distinct estrogen‐regulated promoters generate transcripts encoding the two functionally different human progesterone receptor forms A and B. The EMBO Journal. ,vol. 9, pp. 1603- 1614 ,(1990) , 10.1002/J.1460-2075.1990.TB08280.X
George Wilding, Marc E. Lippman, Edward P. Gelmann, Effects of steroid hormones and peptide growth factors on protooncogene c-fos expression in human breast cancer cells. Cancer Research. ,vol. 48, pp. 802- 805 ,(1988)
Benita S. Katzenellenbogen, Georg C. A. Reiner, Characterization of Estrogen and Progesterone Receptors and the Dissociated Regulation of Growth and Progesterone Receptor Stimulation by Estrogen in MDA–MB–134 Human Breast Cancer Cells Cancer Research. ,vol. 46, pp. 1124- 1131 ,(1986)
JOHN D. NASH, ROBERT F. OZOLS, JOHN F. SMYTH, THOMAS C. HAMILTON, Estrogen and anti-estrogen effects on the growth of human epithelial ovarian cancer in vitro. American Journal of Obstetrics and Gynecology. ,vol. 73, pp. 1009- 1016 ,(1989) , 10.1097/00006250-198906000-00021
John M. Kissane, Eli Robins, The fluorometric measurement of deoxyribonucleic acid in animal tissues with special reference to the central nervous system. Journal of Biological Chemistry. ,vol. 233, pp. 184- 188 ,(1958) , 10.1016/S0021-9258(19)68051-8