Plasmodium falciparum STEVOR phosphorylation regulates host erythrocyte deformability enabling malaria parasite transmission
作者： Anna Bachmann , Catherine Lavazec , Bernina Naissant , Florian Dupuy , Yoann Duffier
DOI: 10.1182/BLOOD-2016-01-690776
关键词:
摘要: Abstract Deformability of Plasmodium falciparum gametocyte-infected erythrocytes (GIEs) allows them to persist for several days in blood circulation and ensure transmission mosquitoes. Here, we investigate the mechanism by which parasite proteins STEVOR (SubTElomeric Variable Open Reading frame) exert changes on GIE deformability. Using microsphiltration method, immunoprecipitation, mass spectrometry, produce evidence that stiffness is dependent cytoplasmic domain interacts with ankyrin complex at erythrocyte skeleton. Moreover, show deformability regulated protein kinase A (PKA)–mediated phosphorylation C-terminal a specific serine residue (S324). Finally, increase induced sildenafil (Viagra) status another independent mechanism. These data provide new insights into mechanisms phosphodiesterase inhibitors may block malaria transmission.
ashpublications.org
bloodjournal.org参考文章(53)
Sandra K. Nilsson, Lauren M. Childs, Caroline Buckee, Matthias Marti, Targeting Human Transmission Biology for Malaria Elimination PLOS Pathogens. ,vol. 11, pp. e1004871- ,(2015) , 10.1371/JOURNAL.PPAT.1004871
Marta Tibúrcio, Francesco Silvestrini, Lucia Bertuccini, Adam Frederik Sander, Louise Turner, Thomas Lavstsen, Pietro Alano, Early gametocytes of the malaria parasite Plasmodium falciparum specifically remodel the adhesive properties of infected erythrocyte surface. Cellular Microbiology. ,vol. 15, pp. 647- 659 ,(2013) , 10.1111/CMI.12062
Julien Duez, John P Holleran, Papa Alioune Ndour, Sasdekumar Loganathan, Pascal Amireault, Olivier Français, Wassim El Nemer, Bruno Le Pioufle, Inês F Amado, Sylvie Garcia, Nathalie Chartrel, Caroline Le Van Kim, Catherine Lavazec, Vicky M Avery, Pierre A Buffet, None, Splenic Retention of Plasmodium falciparum Gametocytes To Block the Transmission of Malaria Antimicrobial Agents and Chemotherapy. ,vol. 59, pp. 4206- 4214 ,(2015) , 10.1128/AAC.05030-14
Ghania Ramdani, Bernina Naissant, Eloise Thompson, Florence Breil, Audrey Lorthiois, Florian Dupuy, Ross Cummings, Yoann Duffier, Yolanda Corbett, Odile Mercereau-Puijalon, Kenneth Vernick, Donatella Taramelli, David A. Baker, Gordon Langsley, Catherine Lavazec, cAMP-Signalling Regulates Gametocyte-Infected Erythrocyte Deformability Required for Malaria Parasite Transmission. PLOS Pathogens. ,vol. 11, ,(2015) , 10.1371/JOURNAL.PPAT.1004815
Anna Bachmann, Judith Anna Marie Scholz, Marthe Janßen, Mo-Quen Klinkert, Egbert Tannich, Iris Bruchhaus, Michaela Petter, A comparative study of the localization and membrane topology of members of the RIFIN, STEVOR and PfMC-2TM protein families in Plasmodium falciparum-infected erythrocytes. Malaria Journal. ,vol. 14, pp. 274- 274 ,(2015) , 10.1186/S12936-015-0784-2
S P Kennedy, S L Warren, B G Forget, J S Morrow, Ankyrin binds to the 15th repetitive unit of erythroid and nonerythroid beta-spectrin. Journal of Cell Biology. ,vol. 115, pp. 267- 277 ,(1991) , 10.1083/JCB.115.1.267
Antonella Pantaleo, Lucia De Franceschi, Emanuela Ferru, Rosa Vono, Franco Turrini, Current knowledge about the functional roles of phosphorylative changes of membrane proteins in normal and diseased red cells. Journal of Proteomics. ,vol. 73, pp. 445- 455 ,(2010) , 10.1016/J.JPROT.2009.08.011
Lionel Blanc, Marcela Salomao, Xinhua Guo, Xiuli An, Walter Gratzer, Narla Mohandas, Control of erythrocyte membrane-skeletal cohesion by the spectrin-membrane linkage. Biochemistry. ,vol. 49, pp. 4516- 4523 ,(2010) , 10.1021/BI1003684
J.E. Blythe, T. Surentheran, P.R. Preiser, STEVOR--a multifunctional protein? Molecular and Biochemical Parasitology. ,vol. 134, pp. 11- 15 ,(2004) , 10.1016/J.MOLBIOPARA.2003.09.011
Sohini Sanyal, Stéphane Egée, Guillaume Bouyer, Sylvie Perrot, Innocent Safeukui, Emmanuel Bischoff, Pierre Buffet, Kirk W. Deitsch, Odile Mercereau-Puijalon, Peter H. David, Thomas J. Templeton, Catherine Lavazec, Plasmodium falciparum STEVOR proteins impact erythrocyte mechanical properties Blood. ,vol. 119, ,(2012) , 10.1182/BLOOD-2011-08-370734