Inhibition of the function of the FcgammaRIIB by a monoclonal antibody to thymic shared antigen-1, a Ly-6 family antigen.
作者： Linna Ding , Ethan M. Shevach
DOI: 10.1046/J.1365-2567.2001.01275.X
关键词:
摘要: Thymic shared antigen-1 (TSA-1) is a member of the Ly-6 family glycosyl-phosphatidylinositol (GPI)-linked proteins. While it has been proposed that TSA-1 may play role in thymic development, physiological ligand for this antigen not identified. Here we report monoclonal antibody (mAb) to TSA-1, generated by immunizing hamster with CD40 (CD40L)-activated B cells, interferes function FcgammaRIIB on splenic cells and B-cell lymphoma cell line, M12, binding TSA same cells. The interaction anti-TSA resulted an inhibition ability cross-link and/or aggregate soluble anti-CD3 or anti-Cbeta T-cell receptor (TCR), leading induction expression CD25 CD69, interleukin (IL)-2 production proliferation naive T Cross-blocking studies mAbs strongly suggested physical association exists between surface activated favour view functional intermolecular these two distinct membrane antigens.
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