Covalent attachment of functional protein to polymer surfaces: a novel one-step dry process.

作者: Christopher MacDonald , Richard Morrow , Anthony S Weiss , Marcela M.M Bilek

DOI: 10.1098/RSIF.2007.1352

关键词:

摘要: The attachment of bioactive protein to surfaces underpins the development biosensors and diagnostic microarrays. We present a surface treatment using plasma immersion ion implantation (PIII) create stable covalent binding sites for functional soya-bean peroxidase (SBP). Fourier transform infrared spectra show that is retained on after boiling in sodium dodecyl sulphate hydroxide, which indicative attachment. activity SBP treated remains high comparison with attached control over course 11 days. Surface plasmon resonance was used coverage close monolayer. describe potential PIII method be as one-step dry process large-scale micro- or nanopatterning.

参考文章(28)
Robert E. Feeney, Gunter Blankenhorn, Henry B.F. Dixon, Carbonyl-amine reactions in protein chemistry. Advances in Protein Chemistry. ,vol. 29, pp. 135- 203 ,(1975) , 10.1016/S0065-3233(08)60412-X
Bell Reuben H, Foley Kevin M, Silane coupling agents ,(1974)
K. Lee, B. Lee, J. Hoepner, L. Economikos, C. Parks, C. Radens, J. Bernstein, P. Kellerman, Plasma immersion ion implantation as an alternative deep trench buried-plate doping technology international conference on ion implantation technology. pp. 460- 463 ,(2000) , 10.1109/IIT.2000.924187
M. Schena, D. Shalon, R. W. Davis, P. O. Brown, Quantitative Monitoring of Gene Expression Patterns with a Complementary DNA Microarray Science. ,vol. 270, pp. 467- 470 ,(1995) , 10.1126/SCIENCE.270.5235.467
Joan P. Y. Ho, Neil J. Nosworthy, Marcela M. M. Bilek, Bee K. Gan, David R. McKenzie, Paul K. Chu, Cris G. dos Remedios, Plasma‐Treated Polyethylene Surfaces for Improved Binding of Active Protein Plasma Processes and Polymers. ,vol. 4, pp. 583- 590 ,(2007) , 10.1002/PPAP.200600182
Neil J Nosworthy, Joan PY Ho, Alexey Kondyurin, David R McKenzie, Marcela MM Bilek, None, The attachment of catalase and poly-l-lysine to plasma immersion ion implantation-treated polyethylene. Acta Biomaterialia. ,vol. 3, pp. 695- 704 ,(2007) , 10.1016/J.ACTBIO.2007.02.005
J. K. Amisha Kamal, Digambar V. Behere, Thermal and conformational stability of seed coat soybean peroxidase. Biochemistry. ,vol. 41, pp. 9034- 9042 ,(2002) , 10.1021/BI025621E
B.K. Gan, N.J. Nosworthy, D.R. McKenzie, C.G. dos Remedios, M.M.M. Bilek, Plasma immersion ion implantation treatment of polyethylene for enhanced binding of active horseradish peroxidase. Journal of Biomedical Materials Research Part A. ,vol. 85, pp. 605- 610 ,(2008) , 10.1002/JBM.A.31612
W.L. Barnes, J.R. Sambles, Guided optical waves in Langmuir-Blodgett films of 22 tricosenoic acid Surface Science Letters. ,vol. 177, pp. 399- 416 ,(1986) , 10.1016/0167-2584(86)90962-X