Changes in urine headspace composition as an effect of strenuous walking

摘要: The present investigation uses proton transfer reaction mass spectrometry (PTR-MS) combined with multivariate and univariate statistical analyses to study potential biomarkers for altered metabolism in urine due strenuous walking. Urine samples, concurrence breath blood were taken from 51 participants (23 controls, 11 type-1 diabetes, 17 type-2 diabetes) during the Dutch endurance walking event, International Four Days Marches. Multivariate analysis allowed discrimination of before after exercise all three groups (control, on out 4 days. highlighted 12 molecular ions contributing this discrimination. Of these, acetic acid is identified as a significant marker effects induced by walking; an increase observed effect Analysis acetone concentration tools resulted different information when compared function exercise, revealing interesting time over In breath, provides immediate snapshot metabolism, whereas urinary will result longer term diffusion processes, providing averaged view metabolism. use PTR-MS measurements monitor exhibited, may be utilized subjects participation events.