Unraveling the mode of binding of the anticancer drug topotecan with dsDNA

作者: Hrishikesh Joshi , Abhigyan Sengupta , Krishna Gavvala , Partha Hazra

DOI: 10.1039/C3RA42462F

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摘要: The binding interactions between antitumor drugs and DNA are of burgeoning interest due to increasing demand in medicinal science. In the present work, we have tried examine mode topotecan (TPT) with DNA. TPT, an eminent anti-cancer drug from Camptothecin family, is found interact Topoisomerase-I inhibits replication process. Steady state, time resolved fluorescence, circular dichroism thermal melting studies been utilized explore TPT synthetic polynucleotides ((dA-dT)15, (dG-dC)15) natural (CT-DNA). double helix has substantiated be principally groove binding. It that even though ground state cationic form (C) binds dsDNA irrespective sequences, emission mainly appears Z*, it attributed intermolecular excited proton transfer (ESPT) reaction surrounding water molecules. However, case (dA-dT)15, profile indicates existence a small population (C*) minor different photophysical behavior (dA-dT)15 compared others narrower deeper than others. exact molecular picture interaction DNAs explored modeling studies.

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