Lunasin attenuates obesity-related inflammation in RAW264.7 cells and 3T3-L1 adipocytes by inhibiting inflammatory cytokine production

摘要: Obesity has become a major threat to public health and is accompanied by chronic low-grade inflammation, which leads various pathological developments. Lunasin, natural seed peptide, exhibits several biological activities, such as anti-carcinogenesis, anti-inflammatory, antioxidant activities. However, the mechanism of action lunasin in obesity-related inflammation not been investigated. The aim this study was explore whether could reduce induced mediators RAW264.7 cells 3T3-L1 adipocytes it attenuate crosstalk between two cell lines. were cultured leptin-containing medium, adipocyte-conditioned medium (Ad-CM), or co-cultured with mimic physiology obesity. data showed that secretion pro-inflammatory cytokine interleukin-1β (IL-1β) inhibited after leptin activation cells. In addition, decreased monocyte chemoattractant protein-1 (MCP-1) IL-1β secretions Ad-CM model. Cytokine MCP-1, IL-6, tumor necrosis factor (TNF)-α, significantly plus lipopolysaccharide stimulation. Subsequently, co-culture refined direct relation them, resulting apparently increased IL-6 levels treatment. adipocytes, also exhibited anti-inflammatory property inhibiting plasminogen activator inhibitor-1, productions stimulated lipopolysaccharide, cell-conditioned medium. This result revealed acts potential agent only macrophages but disrupting these Therefore, suggests intake from diet supplement, for auxiliary prevention therapy inflammatory applications.