Adenovirus E1A renders infected cells sensitive to cytolysis by tumor necrosis factor.

摘要: TNF is a multifunctional protein that secreted by activated macrophages and believed to have antiviral properties. We reported previously certain murine fibroblasts infected with group C human adenoviruses become sensitive cytolysis TNF, 14,700 m.w. (14.7K) encoded the E3 transcription unit protects cells from cytolysis. now report mapping of adenovirus genes induce sensitivity Experiments using hydroxyurea or 1-beta-D-arabinofuranosylcytosine showed conferred an early gene. Further was done infecting double mutants lack both protection function E3-14.7K various other testing for sensitivity. Using this method we found none in regions E1B, E3, E4, VA1-RNA are involved creating TNF-sensitive cellular phenotype. However, did not lyse dl312, mutant lacks 14.7K gene plus region E1A, suggesting E1A proteins required TNF. Mutants dl237 13SWT, which produce only 12S (243R) 13S (289R) proteins, respectively, do render indicating provide function. Finally, E1A-transfected cells, killing, more when thus alone among responsible susceptibility cells.