Expression of inhibitor of apoptosis proteins (IAPs) in rat granulosa cells during ovarian follicular development and atresia.

摘要: The inhibitor of apoptosis proteins (IAPs) constitute a family highly conserved suppressor that was originally identified in baculoviruses. Although IAP homologs have been recently and demonstrated to suppress mammalian cells, their expression role during follicular development atresia are unknown. present study conducted address these questions. Using established vivo models for the induction immature rats, it possible compare immunolocalization X-link protein (Xiap) human protein-2 (Hiap-2), two members family, at defined stages maturation relate differences observed with those cell proliferation [as determined by proliferating nuclear antigen (PCNA) immunohistochemistry situ terminal deoxynucleotidyl transferase-mediated dUTP-biotin end labeling (TUNEL), respectively]. In addition, granulosa DNA were assessed apoptotic fragmentation 3'-end labeling/agarose gel electrophoresis (DNA ladder) Hiap-2 Xiap content Western blot analysis, respectively. both theca cells increased maturation, reaching maximal levels antral stage development. immunoreactivity PCNA, Xiap, decreased markedly atretic (TUNEL-positive) follicles small medium sized development, suggesting may be associated proliferation. Atresia also change intracellular distribution IAPs cells. Biochemical analysis from preantral early indicates extensive minimal content. Gonadotropin treatment suppressed resulting preovulatory stages. gonadotropin withdrawal induced follicles, which is accompanied marked decrease expression. These data suggest involved suppression medium-sized play an important determining fate thus eventual destiny (atresia vs. ovulation).