Practical limits of resolution in confocal and non-linear microscopy.

摘要: Calculated and measured resolution figures are presented for confocal microscopes with different pinhole sizes nonlinear (2-photon second harmonic) microscopes. A modest degree of super-resolution is predicted a microscope but in practice this not achievable fluorescence gives little improvement over widefield. However, practical non-linear do approach their theoretical therefore show no disadvantage relative to spite the longer excitation wavelength.