Gene analysis of K-, H-ras, p53, and retinoblastoma susceptibility genes in human lung cancer cell lines by the polymerase chain reaction/single-strand conformation polymorphism method.
作者： T. Kashii , Y. Mizushima , S. Monno , K. Nakagawa , M. Kobayashi
DOI: 10.1007/BF01202192
关键词:
摘要: In order to know the involvement of multiple gene alterations in pathogenesis human lung cancer, we examined genes K-, H-ras (codons 12, 13, 61), p53(exons 5–9) and retinoblastoma susceptibility (RB)(exons 20–22) using polymerase chain reaction/single-strand conformation polymorphism method 32 cancer cell lines (5 squamous-cell carcinomas, 10 adenocarcinomas, 3 large-cell 14 small-cell carcinomas). 18 non-small-cell lines, were found 4 for K-ras (22%), none (0%), p53 (22%) RB (0%) gene. (SCLC) no or but 6 (43%) (21%) Coincident abnormalities p53, not any those only 2 SCLC lines. No association was observed between these three N-myc amplification. Although above may be involved some extent more factors are required its development.
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