Incidence and Possible Clinical Significance of K- ras Oncogene Activation in Adenocarcinoma of the Human Lung

摘要: 47 tumor samples, 45 of which were obtained at thoracotomy for non-small cell lung cancer examined mutational activation the oncogenes H-ras, K-ras, and N-ras. A novel, highly sensitive assay based on oligonucleotide hybridization following an in vitro amplification step was employed. ras gene mutations present nine 35 adenocarcinomas (all K-ras), two metastases colorectal (1 x 1 N-ras) one adenocarcinoma sample autopsy (H-ras). All K-ras H-ras either position or 2 codon 12, while N-ras mutation 61. The potential clinical significance analyzed using combined results this our earlier study (S. Rodenhuis et al., New Engl. J. Med., 317: 929-935, 1987). Lung with tended to be smaller less likely have spread regional lymph nodes presentation. With a median follow up 10 months, survival data are still immature. None six nonsmokers had only four who stopped smoking more than 5 years before. We conclude that is about third event may direct result carcinogenic ingredients tobacco smoke. Studies involving larger numbers patients required confirm association inverse relation progression.