Identification of an activated c-Ki-ras oncogene in rat liver tumors induced by aflatoxin B1

摘要: Abstract Weanling male Fischer rats were administered 40 intraperitoneal injections of aflatoxin B1 (25 micrograms per animal day) over a 2-month period. This chronic dosing regimen resulted in the sequential formation hyperplastic foci, preneoplastic nodules, and hepatocellular carcinomas all animals treated. The presence transforming DNA sequences was detected by anchorage-independent foci after transfection tumor-derived NIH 3T3 mouse fibroblasts. Transfection genomic isolated from individual tumors eight specific activities ranging 0.05 to 0.2 DNA. Primary transfectant DNAs analyzed Southern blot hybridization with probes homologous c-Ha-ras, c-Ki-ras, N-ras oncogenes. A highly amplified c-Ki-ras oncogene rat origin transformants derived two tested. There no evidence suggest c-Ha-ras or any transformants. Analysis primary liver tumor showed Ki-ras amplification when compared control samples. Increased levels p21 proteins containing activated genes. capable inducing transformed can be taken as that gene has been tumors.